Komal Garde – Ph.D. Preliminary Thesis Defense 05/04/2016 at 11:00 A.M.

JSNN – Komal Garde – Ph.D. Dissertation Preliminary Proposal Defense

Candidate: Komal Garde

Advisor: Dr. Shyam Aravamudhan

Department: Nanoengineering

Date: Wednesday, May 4, 2016

Time:11:00 A.M. – 1:00 P.M.

Location: JSNN Auditorium

2907 E. Gate City Blvd.,
Greensboro, NC 27401

Title: Use of Electrical Stimulation via Nanoelectrodes to Differentiate Adipose Derived Stem Cells to Neural Lineage.”

Abstract:

The application of electrical stimulation (ES) to stem cells is currently being explored as a method to facilitate their differentiation into various cell lineages. The potential of differentiation of adipose-derived stem cells (ADSCs) is an active area of research due to the ubiquitous nature of adipose cells throughout the body, their ease of extraction and rapid expansion. ES of ADSCs can enhance its proliferation and differentiation into neural phenotypes. ES can be done via non-penetrating or penetrating electrodes. Penetrating electrodes offer direct access to the cell’s interior in a minimally invasive fashion, and enhanced cell-nanopillar coupling. Under those circumstances, the loss of electrical signal is minimal. Nanoelectrodes (pillars and fins) of controlled height, diameter, and density were fabricated on silicon using nanofabrication techniques. Preliminary experiments were conducted using PC12 neural and 3T3 fibroblast cells to confirm the ability of the nanoelectrodes to penetrate cells. The objective of this proposed work is to use similar penetrating nanoelectrodes on ADSCs and subsequently apply electrical stimulus without or with minimal exogenous growth factors in order to obtain neural phenotypes. Furthermore, functional validation will also be done by recording the electrical activity in the neural phenotypes.